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Glycoside Hydrolase Family 121
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- Author: ^^^Kiyotaka Fujita^^^
- Responsible Curator: ^^^Shinya Fushinobu^^^
Glycoside Hydrolase Family GH121 | |
Clan | GH-x |
Mechanism | retaining |
Active site residues | not known |
CAZy DB link | |
http://www.cazy.org/GH121.html |
Substrate specificities
This family of glycoside hydrolases contains β-L-arabinobiosidase, which was recently established for HypBA2 from Bifidobacterium longum JCM 1217[1].HypBA2 liberated Arafβ1-2Araf(β-Ara2) from unmodified Arafβ1-2Arafβ1-2Arafβ-Hydroxyproline (Ara3-Hyp) but not Arafα1-3Arafβ1-2Arafβ1-2Arafβ-Hyp(Ara4-Hyp) or Arafβ1-2Arafβ-Hyp (Ara2-Hyp).
Kinetics and Mechanism
HypBA2 is a retaining enzyme.The stereochemical course of the reaction was shown by transglycosylation activity toward 1-alkanols, and following 1H-NMR and 13C-NMR analysis of the methanol-adducted transglycosylation product.
Catalytic Residues
Not known.
Three-dimensional structures
Not known.
Family Firsts
- First stereochemistry determination
- This was determined with HypBA2 enzyme by measurement of glycosyl transfer reactions to methanol and the 1H-NMR and 13C-NMR spectra.
- First catalytic nucleophile identification
- No experimental proof.
- First general acid/base residue identification
- No experimental proof.
- First 3-D structure
- Not known.