Glycoside Hydrolase Family 71

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• Author: Antonielle Vieira Monclaro
• Responsible Curator: Johan Larsbrink

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Glycoside Hydrolase Family GH71
Clan	GH-x
Mechanism	inverting
Active site residues	known
CAZy DB link
https://www.cazy.org/GH71.html

Substrate specificities

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Authors may get an idea of what to put in each field from Curator Approved Glycoside Hydrolase Families. (TIP: Right click with your mouse and open this link in a new browser window...)

In the meantime, please see these references for an essential introduction to the CAZy classification system: [1, 2].

Kinetics and Mechanism

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Catalytic Residues

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Three-dimensional structures

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Family Firsts

First stereochemistry determination

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First catalytic nucleophile identification

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First general acid/base residue identification

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First 3-D structure

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References

3. Zonneveld, B.J.M. (1972) ‘A new type of enzyme, an exo-splitting α-1,3 glucanase from non-induced cultures of Aspergillus nidulans’, Biochimica et Biophysica Acta (BBA) – Enzymology, 258, pp. 541–547. DOI: 10.1016/0005-2744(72)90245-8

4. Imai, K., Kobayashi, M. and Matsuda, K. (1977) ‘Properties of an α-1,3-glucanase from Streptomyces sp. KI-8’, Agricultural and Biological Chemistry, 41, pp. 1889–1895. DOI: 10.1080/00021369.1977.10862782

5. Fuglsang, C.C., Berka, R.M., Wahleithner, J.A., Kauppinen, S., Shuster, J.R., Rasmussen, G., Halkier, T., Dalbøge, H. and Henrissat, B. (2000) ‘Biochemical analysis of recombinant fungal mutanases’, Journal of Biological Chemistry, 275, pp. 2009–2018. DOI: 10.1074/jbc.275.3.2009

6. Villalobos-Duno, H., San-Blas, G., Paulinkevicius, M., Sánchez-Martín, Y. and Nino-Vega, G. (2013) ‘Biochemical characterization of Paracoccidioides brasiliensis α-1,3-glucanase Agn1p, and its functionality by heterologous expression in Schizosaccharomyces pombe’, PLoS ONE, 8, e66853. DOI: 10.1371/journal.pone.0066853

7. Ait-Lahsen, H., Soler, A., Rey, M., De La Cruz, J., Monte, E. and Llobell, A. (2001) ‘An antifungal exo-α-1,3-glucanase (AGN13.1) from the biocontrol fungus Trichoderma harzianum’, Applied and Environmental Microbiology, 67, pp. 5833–5839. DOI: 10.1128/AEM.67.12.5833-5839.2001

8. Dekker, N., Speijer, D., Grün, C.H., Van den Berg, M., De Haan, A. and Hochstenbach, F. (2004) ‘Role of the α-glucanase Agn1p in fission-yeast cell separation’, Molecular Biology of the Cell, 15, pp. 3903–3914. DOI: 10.1091/mbc.E04

9. Mazurkewich, S., Widén, T., Karlsson, H., Evenäs, L., Ramamohan, P., Wohlert, J., Brändén, G. and Larsbrink, J. (2025) ‘Structural and biochemical basis for activity of Aspergillus nidulans α-1,3-glucanases from glycoside hydrolase family 71’, Communications Biology, 8. DOI: 10.1038/s42003-025-08696-3

10. Grün, C.H., Dekker, N., Nieuwland, A.A., Klis, F.M., Kamerling, J.P., Vliegenthart, J.F.G. and Hochstenbach, F. (2006) ‘Mechanism of action of the endo-(1→3)-α-glucanase MutAp from the mycoparasitic fungus Trichoderma harzianum’, FEBS Letters, 580, pp. 3780–3786. DOI: 10.1016/j.febslet.2006.05.062

11. Sinitsyna, O.A., Volkov, P.V., Zorov, I.N., Rozhkova, A.M., Emshanov, O.V., Romanova, Y.M., Komarova, B.S., Novikova, N.S., Nifantiev, N.E. and Sinitsyn, A.P. (2025) ‘Physico-chemical properties and substrate specificity of α-(1→3)-D-glucan degrading recombinant mutanase from Trichoderma harzianum expressed in Penicillium verruculosum’, Applied and Environmental Microbiology, 91. DOI: 10.1128/aem.00226-24

12. Horaguchi, Y., Saitoh, H., Konno, H., Makabe, K. and Yano, S. (2025) ‘Crystal structure of GH71 α-1,3-glucanase Agn1p from Schizosaccharomyces pombe: an enzyme regulating cell division in fission yeast’, Biochemical and Biophysical Research Communications, 766. DOI: 10.1016/j.bbrc.2025.151907