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New to the CAZy classification? Read this first.
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Auxiliary Activity Family 10
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Vincent Eijsink^^^ and ^^^Gustav Vaaje-Kolstad^^^
- Responsible Curator: ^^^Vincent Eijsink^^^
| Auxiliary Activity Family 10 | |
| Clan | none, structurally related to AA9 |
| Mechanism | lytic oxidase |
| Active site residues | mononuclear copper ion |
| CAZy DB link | |
| http://www.cazy.org/AA10.html | |
Substrate specificities
Before the proteins belonging to AA10 were shown to be enzymes, they were known as chitin binding proteins (CBPs), since most characterized proteins had been identified in chitinolytic systems such as that of Serratia marcescens (REF), several Streptomyces species (REFs), ......, and show no other obvious function than just binding the substrate. Thus there exists several papers that have analyzed the binding preferences of AA10s.
Please see these references for an essential introduction to the CAZy classification system: [1, 2].
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First stereochemistry determination
- Content is to be added here.
- First catalytic nucleophile identification
- Content is to be added here.
- First general acid/base residue identification
- Content is to be added here.
- First 3-D structure
- CBP21, the single AA10-type LPMO from the Gram negative bacterium Serratia marcescens. Entry in the protein data bank: [1]
References
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Davies, G.J. and Sinnott, M.L. (2008) Sorting the diverse: the sequence-based classifications of carbohydrate-active enzymes. Biochem. J. (BJ Classic Paper, online only). DOI: 10.1042/BJ20080382
- Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, and Henrissat B. (2009). The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res. 2009;37(Database issue):D233-8. DOI:10.1093/nar/gkn663 |