CAZypedia needs your help! We have many unassigned GH, PL, CE, AA, GT, and CBM pages in need of Authors and Responsible Curators.
Scientists at all career stages, including students, are welcome to contribute to CAZypedia. Read more here, and in the 10th anniversary article in Glycobiology.
New to the CAZy classification? Read this first.
*
Consider attending the 15th Carbohydrate Bioengineering Meeting in Ghent, 5-8 May 2024.

Difference between revisions of "Carbohydrate Binding Module Family 92"

From CAZypedia
Jump to navigation Jump to search
Line 25: Line 25:
  
 
== Functionalities ==  
 
== Functionalities ==  
A carrageenan-specific fluorescent probe was successfully constructed by fusing Cgk16A-CBM92 with a green fluorescent protein. The in situ visualization of carrageenan in red alga Kappaphycus alvarezii was realized by utilizing the fluorescent probe [1].
+
A carrageenan-specific fluorescent probe was successfully constructed by fusing Cgk16A-CBM92 with a green fluorescent protein. The ''in situ'' visualization of carrageenan in red alga ''Kappaphycus alvarezii'' was realized by utilizing the fluorescent probe [1].
  
 
== Family Firsts ==
 
== Family Firsts ==
 
;First Identified
 
;First Identified
The first member Cgk16A-CBM92 is a component of a κ-carrageenase Cgk16A [2], which was discovered from a marine bacterium Wenyingzhuangia aestuarii OF219.  
+
The first member Cgk16A-CBM92 is a component of a κ-carrageenase Cgk16A [2], which was discovered from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219.  
  
 
== References ==
 
== References ==
<biblio>
+
[1] X. Mei, Y. Chang, J. Shen, Y. Zhang, J. Han, C. Xue. (2022). ''Characterization of a novel carrageenan-specific carbohydrate- binding module: a promising tool for the in situ investigation of carrageenan''. ''J. Agric. Food Chem''. 2022;70: 9066-9072. DOI: 10.1021/acs.jafc.2c03139 | PUBMED ID: 35830544 [Mei2022]
#Cantarel2009 pmid=18838391
+
[2] J. Shen, Y. Chang, F. Chen, S. Dong. (2018). Expression and characterization of a kappa-carrageenase from marine bacterium Wenyingzhuangia aestuarii OF219: a biotechnological tool for the depolymerization of kappa-carrageenan. Int. J. Biol. Macromol. 2018;112: 93-100. DOI: 10.1016/j.ijbiomac.2018.01.075 | PUBMED ID: 29355636 [Shen2018]
#DaviesSinnott2008 Davies, G.J. and Sinnott, M.L. (2008) Sorting the diverse: the sequence-based classifications of carbohydrate-active enzymes. ''The Biochemist'', vol. 30, no. 4., pp. 26-32. [https://doi.org/10.1042/BIO03004026 Download PDF version].
+
 
#Boraston2004 pmid=15214846
 
#Hashimoto2006 pmid=17131061
 
#Shoseyov2006 pmid=16760304
 
#Guillen2010 pmid=19908036
 
#Armenta2017 pmid=28547780
 
</biblio>
 
  
 
<!-- Do not delete this Category tag -->
 
<!-- Do not delete this Category tag -->
 
[[Category:Carbohydrate Binding Module Families|CBM092]]
 
[[Category:Carbohydrate Binding Module Families|CBM092]]

Revision as of 01:45, 27 January 2023

Under construction icon-blue-48px.png

This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.


CAZy DB link
http://www.cazy.org/CBM92.html

Ligand specificities

The first and only hereinto characterized member in the CBM92 family is Cgk16A-CBM92 [1]. The CBM could specifically bind to carrageenan and showed no significant difference in the affinity to κ- and ι-carrageenan.

Structural Features

No three-dimensional structure has been solved in this CBM family at present.

Functionalities

A carrageenan-specific fluorescent probe was successfully constructed by fusing Cgk16A-CBM92 with a green fluorescent protein. The in situ visualization of carrageenan in red alga Kappaphycus alvarezii was realized by utilizing the fluorescent probe [1].

Family Firsts

First Identified

The first member Cgk16A-CBM92 is a component of a κ-carrageenase Cgk16A [2], which was discovered from a marine bacterium Wenyingzhuangia aestuarii OF219.

References

[1] X. Mei, Y. Chang, J. Shen, Y. Zhang, J. Han, C. Xue. (2022). Characterization of a novel carrageenan-specific carbohydrate- binding module: a promising tool for the in situ investigation of carrageenan. J. Agric. Food Chem. 2022;70: 9066-9072. DOI: 10.1021/acs.jafc.2c03139 | PUBMED ID: 35830544 [Mei2022] [2] J. Shen, Y. Chang, F. Chen, S. Dong. (2018). Expression and characterization of a kappa-carrageenase from marine bacterium Wenyingzhuangia aestuarii OF219: a biotechnological tool for the depolymerization of kappa-carrageenan. Int. J. Biol. Macromol. 2018;112: 93-100. DOI: 10.1016/j.ijbiomac.2018.01.075 | PUBMED ID: 29355636 [Shen2018]