Glycoside Hydrolase Family 101

• Author: Warren Wakarchuk
• Responsible Curator: Warren Wakarchuk

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Glycoside Hydrolase Family GH101
Clan	GH-x
Mechanism	retaining
Active site residues	known
CAZy DB link
http://www.cazy.org/fam/GH101.html

Substrate specificities

The CAZY GH101 family currently contains proteins from 12 species of bacteria, most of which are commensal human bacteria, though some may also be human pathogens. The substrates are glycoproteins which contain the dissacharride Gal-beta-1,3-GalNAc-alpha-R O-linked glycans on proteins. This glycosylation is a feature of mucin containing proteins.

Kinetics and Mechanism

Retaining mechanism determined by H¹-NMR with the BlGH101 enzyme [1].

Catalytic Residues

Using the enzyme from Streptococcus pnuemoniae the nucleophile was determined as residue D764. Willis et al in preparation [2] The acid/base catalyst in SpGH101 was determined to be E796.

Family Firsts

First sterochemistry determination

Cite some reference here, with a short explanation [3].

This was determined with the BlGH101 enzyme using the H¹-NMR technique.

First catalytic nucleophile identification

This was proposed based on the structure of the SpGH101 and BlGH101 structures, and then experimentally shown in SpGH101 by Willis and co-workers [2].

First general acid/base residue identification

experimentally shown in SpGH101 by Willis and co-workers [2]

First 3-D structure

Determined for SpGH101 by Caines and co-workers [4]

References

5. : 16141207