Glycoside Hydrolase Family 121

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• Author: ^^^Kiyotaka Fujita^^^
• Responsible Curator: ^^^Shinya Fushinobu^^^

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Glycoside Hydrolase Family GH121
Clan	GH-x
Mechanism	retaining
Active site residues	known/not known
CAZy DB link
http://www.cazy.org/GH121.html

Substrate specificities

This family of glycoside hydrolases contains β-L-arabinobiosidase, which was recently established for HypBA2 from Bifidobacterium longum JCM 1217[1].

Kinetics and Mechanism

HypBA2 is a retaining enzyme.by measurement of glycosyl transfer reactions to methanol

Catalytic Residues

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Three-dimensional structures

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Family Firsts

First stereochemistry determination

This was determined with HypBA2 enzyme using the ¹H-NMR and ¹³C-NMR spectra to identify the transglycosylation product β-Ara₂-OMe.

First catalytic nucleophile identification

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First general acid/base residue identification

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First 3-D structure

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References

1. Fujita K, Sakamoto S, Ono Y, Wakao M, Suda Y, Kitahara K, and Suganuma T. (2011). Molecular cloning and characterization of a beta-L-Arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family. J Biol Chem. 2011;286(7):5143-50. DOI:10.1074/jbc.M110.190512 |