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Difference between revisions of "Glycoside Hydrolase Family 121"

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* [[Author]]: ^^^Kiyotaka Fujita^^^
 
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== Substrate specificities ==
 
== Substrate specificities ==
This family of [[glycoside hydrolases]] contains &beta;-L-arabinobiosidase, which was recently established for HypBA2 from ''Bifidobacterium longum'' JCM 1217 <cite>Fujita2011A</cite>. HypBA2 liberates Ara''f''&beta;1-2Ara''f'' (&beta;-Ara<sub>2</sub>) from unmodified Ara''f''&beta;1-2Ara''f''&beta;1-2Ara''f''&beta;-hydroxyproline (Ara<sub>3</sub>-Hyp) but not Ara''f''&alpha;1-3Ara''f''&beta;1-2Ara''f''&beta;1-2Ara''f''&beta;-Hyp (Ara<sub>4</sub>-Hyp) or Ara''f''&beta;1-2Ara''f''&beta;-Hyp (Ara<sub>2</sub>-Hyp). HypBA2 directly liberates &beta;-Ara<sub>2</sub> from hydroxyproline-rich glycoproteins (HRGPs) such as carrot extensin and potato lectin. The family members are only found from prokaryote genomes, such as bacteria and actinomycetes.   
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This family of [[glycoside hydrolases]] contains &beta;-L-arabinobiosidases, as demonstrated for HypBA2 from ''Bifidobacterium longum'' JCM 1217 <cite>Fujita2011A</cite>. HypBA2 liberates the disaccharide Ara''f''&beta;1-2Ara''f'' (&beta;-Ara<sub>2</sub>) from unmodified Ara''f''&beta;1-2Ara''f''&beta;1-2Ara''f''&beta;-hydroxyproline (Ara<sub>3</sub>-Hyp) but not Ara''f''&alpha;1-3Ara''f''&beta;1-2Ara''f''&beta;1-2Ara''f''&beta;-Hyp (Ara<sub>4</sub>-Hyp) or Ara''f''&beta;1-2Ara''f''&beta;-Hyp (Ara<sub>2</sub>-Hyp). HypBA2 directly liberates &beta;-Ara<sub>2</sub> from hydroxyproline-rich glycoproteins (HRGPs) such as carrot extensin and potato lectin. The family members are only found from prokaryote genomes, such as bacteria and actinomycetes.   
  
 
== Kinetics and Mechanism ==
 
== Kinetics and Mechanism ==
HypBA2 is a retaining enzyme. The stereochemical course of the reaction was shown by transglycosylation activity toward 1-alkanols, such as methanol, and produced Ara''f''&beta;1-2Ara''f''&beta;-Me was identified by <sup>1</sup>H-NMR and <sup>13</sup>C-NMR analysis.
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HypBA2 is a retaining enzyme. The stereochemical course of the reaction was shown by transglycosylation activity toward 1-alkanols, such as methanol; the resulting Ara''f''&beta;1-2Ara''f''&beta;-Me was identified by <sup>1</sup>H-NMR and <sup>13</sup>C-NMR analysis.
  
 
== Catalytic Residues ==
 
== Catalytic Residues ==

Revision as of 13:33, 12 November 2012

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Glycoside Hydrolase Family GH121
Clan none
Mechanism retaining
Active site residues not known
CAZy DB link
http://www.cazy.org/GH121.html


Substrate specificities

This family of glycoside hydrolases contains β-L-arabinobiosidases, as demonstrated for HypBA2 from Bifidobacterium longum JCM 1217 [1]. HypBA2 liberates the disaccharide Arafβ1-2Araf (β-Ara2) from unmodified Arafβ1-2Arafβ1-2Arafβ-hydroxyproline (Ara3-Hyp) but not Arafα1-3Arafβ1-2Arafβ1-2Arafβ-Hyp (Ara4-Hyp) or Arafβ1-2Arafβ-Hyp (Ara2-Hyp). HypBA2 directly liberates β-Ara2 from hydroxyproline-rich glycoproteins (HRGPs) such as carrot extensin and potato lectin. The family members are only found from prokaryote genomes, such as bacteria and actinomycetes.

Kinetics and Mechanism

HypBA2 is a retaining enzyme. The stereochemical course of the reaction was shown by transglycosylation activity toward 1-alkanols, such as methanol; the resulting Arafβ1-2Arafβ-Me was identified by 1H-NMR and 13C-NMR analysis.

Catalytic Residues

Not known.

Three-dimensional structures

Not known.

Family Firsts

First stereochemistry determination
This was determined with HypBA2 enzyme by measurement of glycosyl transfer reactions to methanol and the 1H-NMR and 13C-NMR spectra [1].
First catalytic nucleophile identification
No experimental proof.
First general acid/base residue identification
No experimental proof.
First 3-D structure
Not known.

References

  1. Fujita K, Sakamoto S, Ono Y, Wakao M, Suda Y, Kitahara K, and Suganuma T. (2011). Molecular cloning and characterization of a beta-L-Arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family. J Biol Chem. 2011;286(7):5143-50. DOI:10.1074/jbc.M110.190512 | PubMed ID:21149454 [Fujita2011A]