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• Glycoside Hydrolase Family 1
  ...iciently reactive not to require the acid catalyst, while the role of base catalyst is played by exogenous ascorbate, which binds to the glycosyl enzyme <cite> ...apart in sequence and located at the C-terminal ends of β-strands 4 (acid/base) and 7 (nucleophile) <cite>10</cite>.
  5 KB (726 words) - 18:10, 27 November 2012
• Glycoside Hydrolase Family 33
  ...eophilic by an invariant glutamate, Glu230 for TcTS, that serves as a base catalyst for deprotonation/reprotonation of the tyrosine hydroxyl. See Chan et al <c ===Acid Base Catalyst===
  9 KB (1,152 words) - 17:59, 4 January 2023
• Glycoside Hydrolase Family 130
  ...osphate in phosphorylases, but has two Glu residues acting as general base catalyst <cite>Cuskin2015 Nihira2015</cite>. These residues could activate catalytic ...ase from ''Dyadobacter fermentans'', Glu224 and Glu265 act as general base catalyst <cite>Nihira2015</cite> to activate catalytic water.
  5 KB (684 words) - 14:15, 18 December 2021
• Glycoside Hydrolase Family 134
  ...r Glu45 as a [[general acid]] catalyst and for Asp57 as a [[general base]] catalyst <cite>Jin2016</cite>. These roles were supported by mutagenesis with the co ;First [[general base]] residue identification: Asp57 in ''Ss''GH134 by structural study supporte
  4 KB (561 words) - 14:18, 18 December 2021
• Glycoside Hydrolase Family 11
  ...e the changes that occur upon repositioning or removing the [[general acid/base]] residue reveal its contributions <cite>7Lawson</cite>. ;First [[general acid/base]] residue identification: ''Bacillus circulans'' endo-xylanase Bcx by rescu
  3 KB (451 words) - 09:40, 29 July 2015
• Glycoside Hydrolase Family 102
  ...the GH102 enzymes are thought to possess a single catalytic [[general acid/base]] residue. This residue in ''E. coli'' MltA has been identified as Asp308 ...oton of the [[intermediate]] involving Asp308 which now serves as the base catalyst leading to nucleophilic attack and the formation of 1,6-anhydromuramic acid
  5 KB (723 words) - 14:15, 18 December 2021
• Catalytic nucleophile
  ...zyme is hydrolyzed by water, with the other residue now acting as a base catalyst deprotonating the water molecule as it attacks. At the completion of the ca ...ccelerated. On the other hand the deglyclosylation step requires [[general base]] catalysis and remains retarded, leading to accumulation of the glycosyl e
  6 KB (897 words) - 19:26, 5 August 2015
• Glycoside Hydrolase Family 34
  ...lent sialyl-enzyme intermediate on a Tyrosine residue. This is followed by base-catalysed attack of water on the sialyl-enzyme intermediate, releasing Neu5 ...the second step as explained in the CAZy [[Lexicon]] under [[General acid/base]].
  7 KB (1,025 words) - 18:00, 4 January 2023
• Glycoside Hydrolase Family 65
  ...as in [[inverting]] [[glycoside hydrolases]]. The possibility of [[general base]] assistance in the phosphorylases of this family, via a histidine residue ...vis'' maltose phosphorylase) may assist catalysis by acting as a [[general base]] <cite>Egloff2001</cite>, but no experimental evidence has yet been provid
  7 KB (917 words) - 14:18, 18 December 2021
• Glycoside Hydrolase Family 186
  ...has unique long proton transfer pathway from nucleophilic water to general base. General acid and base of EcOpgD are D388 and D300, respectively<cite>MotouchiEc2023</cite>.
  5 KB (637 words) - 02:41, 25 January 2024
• Glycoside Hydrolase Family 136
  ...and TnX) revealed a direct hydrogen bond between the [[general acid/base]] catalyst and the O1 atom. This observation suggests that a direct proton transfer me For LnbX, the [[catalytic nucleophile]] and the catalytic [[general acid/base]] are Asp418 and Asp411, respectively.
  4 KB (566 words) - 12:57, 25 June 2023
• Glycoside Hydrolase Family 95
  ...u566 and Asn423 have been identified as the [[general acid]] and [[general base]] residues, respectively <cite>Nagae2007</cite>. Glu566 is hydrogen-bonded ;First [[general base]] residue identification:1,2-&alpha;-L-Fucosidase from ''Bifidobacterium bi
  6 KB (915 words) - 14:16, 18 December 2021
• Glycoside Hydrolase Family 42
  ...more sensitive to the inhibitor <cite>11</cite>. The [[general acid/base]] catalyst was first identified as Glu157 in the β-galactosidase from ''A. acidocalda ...mes present the catalytic dyad at the C-terminal ends of β-strands 4 (acid/base) and 7 (nucleophile) within a domain showing a classical (&alpha;/β)<sub>8
  5 KB (616 words) - 14:16, 18 December 2021
• Phosphorylases
  ...-like [[transition state]]s, as shown below. The reaction occurs with acid/base assistance from a suitably positioned carboxylate residue. This mechanism h ...nzyme is cleaved by phosphate, with the other residue now acting as a base catalyst deprotonating the phosphate as it attacks. Each step passes through an [[ox
  8 KB (986 words) - 14:20, 18 December 2021
• Glycoside hydrolases
  ...ains both retaining and inverting enzymes; a glutamate acts as a [[general base]] in inverting members, whereas an aspartate likely acts as a [[catalytic n ...below. The reaction typically occurs with [[general acid]] and [[general base]] assistance from two amino acid side chains, normally glutamic or aspartic
  14 KB (1,972 words) - 14:29, 17 December 2023
• Glycoside Hydrolase Family 127
  ...was suggested to act as the nucleophile. Glu322 is possibly the acid/base catalyst. A possible reaction mechanism involving the cysteine residue as the nucleo ;First [[general acid/base]] residue identification:
  4 KB (527 words) - 14:18, 18 December 2021
• Glycoside Hydrolase Family 116
  ...osyl-enzyme [[intermediate]] and MS/MS analysis. The [[general acid/base]] catalyst role was assigned to Asp462 through mechanistic analysis of a mutant at tha ...up> is bound to a site within the same loop that contains the general acid/base.
  6 KB (747 words) - 14:20, 18 December 2021
• Glycoside Hydrolase Family 117
  ...logous Asp-His dyad has been similarly reported to act as the general base catalyst in the retaining mechanism of select [[GH3]] members <cite>Litzinger2010</c ...alignments the catalytic residues have been predicted to be Asp-90 as the base and His-302 as the acid ''Bp''GH117 numbering) <cite>Hehemann2012</cite>.
  5 KB (742 words) - 14:14, 18 December 2021
• Glycoside Hydrolase Family 2
  ...the binding of the cation in the active site such that it places the acid/base residue appropriately. Others, such as the human β-glucuronidase, have no ...e above labeling study <cite>Gebler1992</cite>. The [[general acid/base]] catalyst was then identified as Glu461 by re-interpretation <cite>Gebler1992</cite>
  6 KB (823 words) - 16:35, 23 February 2019
• Glycoside Hydrolase Family 66
  ...d/base]] is glutamic acid. Asp385 and Glu453 are nucleophile and acid/base catalyst, respectively, in Dex from ''Streptococcus mutans'' (''Sm''Dex) <cite>Nsuzu ;First [[general acid/base]] residue identification: ''Sm''Dex and ''Ps''Dex by structural study <cite
  6 KB (819 words) - 14:18, 18 December 2021

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