Glycoside Hydrolase Family 6

• Author: ^^^Gideon Davies^^^
• Responsible Curator: ^^^Gideon Davies^^^

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Glycoside Hydrolase Family GH6
Clan	none
Mechanism	inverting
Active site residues	acid known, base debated
CAZy DB link
http://www.cazy.org/fam/GH6.html

Substrate specificities

Endoglucanases (EC 3.2.1.4) and cellobiohydrolases (EC 3.2.1.91)only. Both classes of enzyme active on cellulose / beta 1,4 glucans.

Kinetics and Mechanism

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Catalytic Residues

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Three-dimensional structures

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Family Firsts

First sterochemistry determination

Cite some reference here, with a short (1-2 senetence) explanation [1].

First catalytic nucleophile identification

Cite some reference here, with a short (1-2 senetence) explanation [2].

First general acid/base residue identification

Cite some reference here, with a short (1-2 senetence) explanation [3].

First 3-D structure

The Trichoderma reesei (the organism now known as Hypocrea jecorina) cellobiohydrolase II by the Jones group [4].

References

2. Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. DOI: 10.1021/cr00105a006

3. He S and Withers SG. (1997). Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile. J Biol Chem. 1997;272(40):24864-7. DOI:10.1074/jbc.272.40.24864 |
4. Rouvinen J, Bergfors T, Teeri T, Knowles JK, and Jones TA. (1990). Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei. Science. 1990;249(4967):380-6. DOI:10.1126/science.2377893 |
5. Robert V. Stick and Spencer J. Williams. (2009) Carbohydrates. Elsevier Science.

[[Category:Glycoside Hydrolase Families|GHnnn]]