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I am an Associate Professor at Laboratory of Enzymology in Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo located in Tokyo, Japan. Raised in Kure, Hiroshima, Japan. I obtained Ph.D degree in Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo in 1999. My research interests concern structure and function of enzymes, mainly those of Carbohydrate-Active enZymes. My home page is here. I contributed to three-dimensional structure determination of

• GH1 Phanerochaete chrysosporium β-glucosidase 1B (BGL1B) [1]
• GH3 Kluyveromyces marxianus β-glucosidase (KmBglI) [2]
• GH8 Bacillus halodurans reducing-end xylose-releasing exo-oligoxylanase (Rex) [3]
• GH10 Clostridium stercorarium xylanase B (XynB) [4]
• GH11 Aspergillus kawachii xylanase C (XynC) [5]
• GH42 Thermus thermophilus β-galactosidase (A4-β-Gal) Family First [6]
• GH54 Aspergillus kawachii α-L-arabinofuranosidase B (AkAbfB) Family First plus identification of CBM42 [7]
• GH55 Phanerochaete chrysosporium β-1,3-glucanase (Lam55A) Family First [8]
• GH57 Thermococcus litoralis 4-α-glucanotransferase (TLGT) Family First [9]
• GH94 Vibrio proteolyticus chitobiose phosphorylase (ChBP) Family First [10]
• GH94 Cellvibrio gilvus cellobiose phosphorylase (CBP) [11]
• GH101 Bifidobacterium longum endo-α-N-acetylgalactosaminidase (EngBF) [12]
• GH112 Bifidobacterium longum galacto-N-biose/lacto-N-biose I phosphorylase (GLNBP) Family First [13]
• PL20 Trichoderma reesei endo-β-1,4-glucuronan lyase (TrGL) Family First [14]
• CBM28 in Clostridium josui Cel5A (CjCBM28) [15]

1. Nijikken Y, Tsukada T, Igarashi K, Samejima M, Wakagi T, Shoun H, and Fushinobu S. Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium. FEBS Lett 2007 Apr 3; 581(7) 1514-20. doi:10.1016/j.febslet.2007.03.009 pmid:17376440. PubMed HubMed [Nijikken2007]
2. Yoshida E, Hidaka M, Fushinobu S, Koyanagi T, Minami H, Tamaki H, Kitaoka M, Katayama T, Kumagai H. Role of a PA14 domain in determining substrate specificity of a glycoside hydrolase family 3 β-glucosidase from Kluyveromyces marxianus. Biochem J. 2010 Oct 1;431(1):39-49.

   [Yoshida2010]

   Note: Due to a problem with PubMed data, this reference is not automatically formatted. Please see these links out: DOI:10.1042/BJ20100351 PMID: 20662765

3. Fushinobu S, Hidaka M, Honda Y, Wakagi T, Shoun H, and Kitaoka M. Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125. J Biol Chem 2005 Apr 29; 280(17) 17180-6. doi:10.1074/jbc.M413693200 pmid:15718242. PubMed HubMed [Fushinobu2005]
4. Nishimoto M, Fushinobu S, Miyanaga A, Kitaoka M, and Hayashi K. Molecular anatomy of the alkaliphilic xylanase from Bacillus halodurans C-125. J Biochem 2007 May; 141(5) 709-17. doi:10.1093/jb/mvm072 pmid:17383976. PubMed HubMed [Nishimoto2007]
5. Fushinobu S, Ito K, Konno M, Wakagi T, and Matsuzawa H. Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH. Protein Eng 1998 Dec; 11(12) 1121-8. pmid:9930661. PubMed HubMed [Fushinobu1998]
6. Hidaka M, Fushinobu S, Ohtsu N, Motoshima H, Matsuzawa H, Shoun H, and Wakagi T. Trimeric crystal structure of the glycoside hydrolase family 42 beta-galactosidase from Thermus thermophilus A4 and the structure of its complex with galactose. J Mol Biol 2002 Sep 6; 322(1) 79-91. pmid:12215416. PubMed HubMed [Hidaka2002]
7. Miyanaga A, Koseki T, Matsuzawa H, Wakagi T, Shoun H, and Fushinobu S. Crystal structure of a family 54 alpha-L-arabinofuranosidase reveals a novel carbohydrate-binding module that can bind arabinose. J Biol Chem 2004 Oct 22; 279(43) 44907-14. doi:10.1074/jbc.M405390200 pmid:15292273. PubMed HubMed [Miyanaga2004]
8. Ishida T, Fushinobu S, Kawai R, Kitaoka M, Igarashi K, and Samejima M. Crystal structure of glycoside hydrolase family 55 {beta}-1,3-glucanase from the basidiomycete Phanerochaete chrysosporium. J Biol Chem 2009 Apr 10; 284(15) 10100-9. doi:10.1074/jbc.M808122200 pmid:19193645. PubMed HubMed [Ishida2009]
9. Imamura H, Fushinobu S, Yamamoto M, Kumasaka T, Jeon BS, Wakagi T, and Matsuzawa H. Crystal structures of 4-alpha-glucanotransferase from Thermococcus litoralis and its complex with an inhibitor. J Biol Chem 2003 May 23; 278(21) 19378-86. doi:10.1074/jbc.M213134200 pmid:12618437. PubMed HubMed [Imamura2003]
10. Hidaka M, Honda Y, Kitaoka M, Nirasawa S, Hayashi K, Wakagi T, Shoun H, and Fushinobu S. Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold. Structure 2004 Jun; 12(6) 937-47. doi:10.1016/j.str.2004.03.027 pmid:15274915. PubMed HubMed [Hidaka2004]
11. Hidaka M, Kitaoka M, Hayashi K, Wakagi T, Shoun H, and Fushinobu S. Structural dissection of the reaction mechanism of cellobiose phosphorylase. Biochem J 2006 Aug 15; 398(1) 37-43. doi:10.1042/BJ20060274 pmid:16646954. PubMed HubMed [Hidaka2006]
12. Suzuki R, Katayama T, Kitaoka M, Kumagai H, Wakagi T, Shoun H, Ashida H, Yamamoto K, and Fushinobu S. Crystallographic and mutational analyses of substrate recognition of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum. J Biochem 2009 Sep; 146(3) 389-98. doi:10.1093/jb/mvp086 pmid:19502354. PubMed HubMed [Suzuki2009]
13. Hidaka M, Nishimoto M, Kitaoka M, Wakagi T, Shoun H, and Fushinobu S. The crystal structure of galacto-N-biose/lacto-N-biose I phosphorylase: a large deformation of a TIM barrel scaffold. J Biol Chem 2009 Mar 13; 284(11) 7273-83. doi:10.1074/jbc.M808525200 pmid:19124470. PubMed HubMed [Hidaka2009]
14. Konno N, Ishida T, Igarashi K, Fushinobu S, Habu N, Samejima M, and Isogai A. Crystal structure of polysaccharide lyase family 20 endo-beta-1,4-glucuronan lyase from the filamentous fungus Trichoderma reesei. FEBS Lett 2009 Apr 17; 583(8) 1323-6. doi:10.1016/j.febslet.2009.03.034 pmid:19306878. PubMed HubMed [Konno2009]
15. Tsukimoto K, Takada R, Araki Y, Suzuki K, Karita S, Wakagi T, Shoun H, Watanabe T, and Fushinobu S. Recognition of cellooligosaccharides by a family 28 carbohydrate-binding module. FEBS Lett 2010 Mar 19; 584(6) 1205-11. doi:10.1016/j.febslet.2010.02.027 pmid:20159017. PubMed HubMed [Tsukimoto2010]