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Difference between revisions of "Glycoside Hydrolase Family 127"
| Line 29: | Line 29: | ||
== Substrate specificities == | == Substrate specificities == | ||
| − | + | This family of [[glycoside hydrolases]] contains β-L-arabinofuranosidase, which was recently established for HypBA1 from ''Bifidobacterium longum'' JCM 1217<cite>Fujita2011B</cite>. | |
== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
| Line 41: | Line 41: | ||
== Family Firsts == | == Family Firsts == | ||
| − | ;First stereochemistry determination: | + | ;First stereochemistry determination: This was determined with HypBA1 enzyme using the <sup>1</sup>H-NMR and <sup>13</sup>C-NMR spectra to identify the transglycosylation product β-Ara-OMe. |
;First catalytic nucleophile identification: Content is to be added here. | ;First catalytic nucleophile identification: Content is to be added here. | ||
;First general acid/base residue identification: Content is to be added here. | ;First general acid/base residue identification: Content is to be added here. | ||
| Line 48: | Line 48: | ||
== References == | == References == | ||
<biblio> | <biblio> | ||
| − | # | + | #Fujita2011B Fujita K, Takashi Y, Obuchi E, Kitahara K, Suganuma T.(2011)Characterization of a novel β-L-Arabinofuranosidase in Bifidobacterium longum: functional elucidation of A DUF1680 family member. J Biol Chem. 286(44), 38079-85.[http://www.ncbi.nlm.nih.gov/pubmed/21914802] |
| − | |||
</biblio> | </biblio> | ||
[[Category:Glycoside Hydrolase Families|GH127]] | [[Category:Glycoside Hydrolase Families|GH127]] | ||
Revision as of 22:31, 26 April 2012
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Kiyotaka Fujita^^^
- Responsible Curator: ^^^Shinya Fushinobu^^^
| Glycoside Hydrolase Family GH127 | |
| Clan | GH-x |
| Mechanism | retaining |
| Active site residues | not known |
| CAZy DB link | |
| http://www.cazy.org/GH127.html | |
Substrate specificities
This family of glycoside hydrolases contains β-L-arabinofuranosidase, which was recently established for HypBA1 from Bifidobacterium longum JCM 1217[1].
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First stereochemistry determination
- This was determined with HypBA1 enzyme using the 1H-NMR and 13C-NMR spectra to identify the transglycosylation product β-Ara-OMe.
- First catalytic nucleophile identification
- Content is to be added here.
- First general acid/base residue identification
- Content is to be added here.
- First 3-D structure
- Content is to be added here.
References
-
Fujita K, Takashi Y, Obuchi E, Kitahara K, Suganuma T.(2011)Characterization of a novel β-L-Arabinofuranosidase in Bifidobacterium longum: functional elucidation of A DUF1680 family member. J Biol Chem. 286(44), 38079-85.[1]