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Difference between revisions of "Glycoside Hydrolase Family 80"
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== Substrate specificities == | == Substrate specificities == | ||
| − | Glycoside hydrolases of family 80 include bacterial proteins. They were characterized from proteobacteria <cite>Park1999</cite> of from species belonging to the Bacteroidetes/Chlorobi group <cite>Matsuda2001 Yi2004</cite>. | + | Glycoside hydrolases of family 80 include bacterial proteins. They were characterized from proteobacteria <cite>Park1999</cite> of from species belonging to the Bacteroidetes/Chlorobi group <cite>Matsuda2001 Yi2004</cite>. They are beta-1,4-chitosanases with endo-splitting activity. Chitin or cellulose are not hydrolyzed <cite>Park1999 Yi2004</cite>. Chitosan hexamer (GlcN)<sub>6</sub> is preferentially hydrolyzed into two trimeric molecules <cite>Shimono2002</cite>. |
| − | |||
new sentence here<cite>Tremblay2000 Sigrist2010</cite> | new sentence here<cite>Tremblay2000 Sigrist2010</cite> | ||
Revision as of 09:41, 14 July 2011
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author:
- Responsible Curator: ^^^Ryszard Brzezinski^^^
| Glycoside Hydrolase Family GH80 | |
| Clan | GH-I |
| Mechanism | not determined |
| Active site residues | inferred |
| CAZy DB link | |
| http://www.cazy.org/GH80.html | |
Substrate specificities
Glycoside hydrolases of family 80 include bacterial proteins. They were characterized from proteobacteria [1] of from species belonging to the Bacteroidetes/Chlorobi group [2, 3]. They are beta-1,4-chitosanases with endo-splitting activity. Chitin or cellulose are not hydrolyzed [1, 3]. Chitosan hexamer (GlcN)6 is preferentially hydrolyzed into two trimeric molecules [4]. new sentence here[5, 6]
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First primary sequence determination
- Chitosanase ChoA from Matsuebacter chitosanotabidus 3001 (now Mitsuaria chitosanitabida) [1, 7]
- First stereochemistry determination
- Cite some reference here, with a short (1-2 sentence) explanation .
- First catalytic nucleophile identification
- Cite some reference here, with a short (1-2 sentence) explanation .
- First general acid/base residue identification
- Cite some reference here, with a short (1-2 sentence) explanation .
- First 3-D structure
- Cite some reference here, with a short (1-2 sentence) explanation.
References
<biblio>
- Park1999 pmid=10542164
- Matsuda2001 Matsuda, Y., Iida, I., Shinogi, T., Kakutani, K., Nonomura, T., Toyoda, H. (2001) In vitro suppression of mycelial growth of Fusarium oxysporum by extracellular chitosanase of Sphingobacterium multivorum and cloning of the chitosanase gene csnSM1. J. Gen. Plant Pathol. 67, 318-324.
- Yi2004 Yi, J.-H., Jang, H.-K., Lee, S.-J., Lee, K.-E., Choi, S.-G. (2004) Purification and properties of chitosanase from chitinolytic beta-Proteobacterium KNU3. J. Microbiol. Biotechnol. 14, 337-343.
- Shimono2002 pmid=11754739
- Tremblay2000 pmid=11068683
- Sigrist2010 pmid=19858104
- Amakata2005 pmid=16166689