A big milestone: 100 Glycoside Hydrolase Family pages have been completed in CAZypedia - read more on the News page!
Glycoside Hydrolase Family 5
Responsible Curator as essentially complete. CAZypedia is a living document, so further improvement of this page is still possible. If you would like to suggest an addition or correction, please contact the page's Responsible Curator directly by e-mail, or using this form.
|Glycoside Hydrolase Family GH5|
|Active site residues||known|
|CAZy DB link|
GH5 is one of the largest of all CAZy glycoside hydrolase families. Previously known as "cellulase family A" [1, 2], a variety of specificities are now known in this family, notably endoglucanase (cellulase) and endomannanase, as well as exoglucanases, exomannanases and β-glucosidase and β-mannosidase. Other activities include 1,6-galactanase, 1,3-mannanase, 1,4-xylanase, endoglycoceramidase, as well as high specificity xyloglucanases. Family GH5 enzymes are found widely distributed across Archae, bacteria and eukaryotes, notably fungi and plants. There are no known human enzymes in GH5. Following the reclassification of a number of GH5 members into GH30 , a GH5 subfamily classification has been presented that delineates members into a number of monospecific and polyspecific clades . A similar subfamily classification was previously devised for GH13 to aid functional prediction .
Kinetics and Mechanism
GH5 enzymes use the classical Koshland double-displacement mechanism and the two catalytic residues (catalytic nucleophile and general acid/base) are known to be glutamates found at the C-terminal ends of β-strands 4 (acid/base) and 7 (nucleophile) [7, 8].
Three-dimensional structures are available for a very large number of Family GH5 enzymes, the first solved being that of the Clostridium thermocellum endoglucanase CelC . As members of Clan GH-A they have a classical (α/β)8 TIM barrel fold with the two key active site glutamic acids being approximately 200 residues apart in sequence and located at the C-terminal ends of β-strands 4 (acid/base) and 7 (nucleophile) [7, 8].
With so many 3D structures in this family, covering many specificities it is clearly hard to pick out notable structural papers. The Bacillus agaradhaerens Cel5A has been extensively studied, notably in the trapping of enzymatic snapshots along the reaction coordinate  but also as a testbed for glycosidase inhibitor design as crystals often diffract to atomic resolution (for example ). The reaction coordinate work on the endoglucanases (thus working on gluco-configured substrates) shows that the substrate binds in 1S3 conformation with the glycosyl enzyme intermediate in 4C1 chair conformation implying catalysis via a near 4H3 half-chair transition state.
By analogy with family GH26 mannnanases  and family GH2 β-mannosidases  it would seem likely that GH5 mannanases use a different conformational itinerary to their glucosidase relatives, likely via a 1S5-OS2 glycosylation pathway and thus via a B2,5 (near) transition-state although direct evidence in this family is limited . An interesting dissection of mannan-degrading enzyme systems has been provided by work in the Gilbert group on the diverse GH5 and GH26 mannanases in Cellvibrio japonicus(see for example [15, 16, 17]).
The Rhodococcal endoglycoceramidase II (EGC) in this family has found application in the chemoenzymatic synthesis of ceramide derivatives . In 2007 the first 3-D structure of a highly specific GH5 xyloglucanase was reported ; this enzyme makes kinetically productive interactions with both xylose and galactose substituents, as reflected in both a high specific activity on xyloglucan and the kinetics of a series of aryl glycosides.
- First sterochemistry determination
- The curator believes this to be the 1H NMR stereochemical determination for EGZ from Erwinia chrysanthemi . GH5 enzymes were also in the comprehensive Gebler study .
- First catalytic nucleophile identification
- Trapped using the classical Withers 2-fluoro method, here with 2',4'-dinitrophenyl 2-deoxy-2-fluoro-β-D-cellobioside, reported in Wang and Withers in 1993 .
- First general acid/base identification
- Several mutagenesis papers has alluded to the importance of a conserved glutamate- one that both Dominguez  and Ducros  correctly postulated as the catalytic acid when the 3-D structures were determined.
- First 3-D structure
- The first 3D structures in family GH5 was an endoglucanase (cellulase) from Clostridium thermocellum reported by the Alzari in 1995 (in a paper which also reported a family GH10 xylanase structure and the similarities between them) . Subsequently, Ducros and colleagues reported the Clostridium cellulolyticum Cel5A also in 1995 .
- Henrissat B, Claeyssens M, Tomme P, Lemesle L, and Mornon JP. Cellulase families revealed by hydrophobic cluster analysis. Gene. 1989 Sep 1;81(1):83-95.
- Gilkes NR, Henrissat B, Kilburn DG, Miller RC Jr, and Warren RA. Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. Microbiol Rev. 1991 Jun;55(2):303-15.
- St John FJ, González JM, and Pozharski E. Consolidation of glycosyl hydrolase family 30: a dual domain 4/7 hydrolase family consisting of two structurally distinct groups. FEBS Lett. 2010 Nov 5;584(21):4435-41. DOI:10.1016/j.febslet.2010.09.051 |
- Aspeborg H, Coutinho PM, Wang Y, Brumer H 3rd, and Henrissat B. Evolution, substrate specificity and subfamily classification of glycoside hydrolase family 5 (GH5). BMC Evol Biol. 2012 Sep 20;12:186. DOI:10.1186/1471-2148-12-186 |
- Stam MR, Danchin EG, Rancurel C, Coutinho PM, and Henrissat B. Dividing the large glycoside hydrolase family 13 into subfamilies: towards improved functional annotations of alpha-amylase-related proteins. Protein Eng Des Sel. 2006 Dec;19(12):555-62. DOI:10.1093/protein/gzl044 |
- Barras F, Bortoli-German I, Bauzan M, Rouvier J, Gey C, Heyraud A, and Henrissat B. Stereochemistry of the hydrolysis reaction catalyzed by endoglucanase Z from Erwinia chrysanthemi. FEBS Lett. 1992 Mar 30;300(2):145-8.
- Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, and Davies G. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc Natl Acad Sci U S A. 1996 May 28;93(11):5674.
- Jenkins J, Lo Leggio L, Harris G, and Pickersgill R. Beta-glucosidase, beta-galactosidase, family A cellulases, family F xylanases and two barley glycanases form a superfamily of enzymes with 8-fold beta/alpha architecture and with two conserved glutamates near the carboxy-terminal ends of beta-strands four and seven. FEBS Lett. 1995 Apr 10;362(3):281-5.
- Davies GJ, Mackenzie L, Varrot A, Dauter M, Brzozowski AM, Schülein M, and Withers SG. Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase. Biochemistry. 1998 Aug 25;37(34):11707-13. DOI:10.1021/bi981315i |
- Varrot A, Tarling CA, Macdonald JM, Stick RV, Zechel DL, Withers SG, and Davies GJ. Direct observation of the protonation state of an imino sugar glycosidase inhibitor upon binding. J Am Chem Soc. 2003 Jun 25;125(25):7496-7. DOI:10.1021/ja034917k |
- Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabó L, Stoll D, Withers SG, and Davies GJ. Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state. Angew Chem Int Ed Engl. 2002 Aug 2;41(15):2824-7. DOI:10.1002/1521-3773(20020802)41:15<2824::AID-ANIE2824>3.0.CO;2-G |
- Tailford LE, Offen WA, Smith NL, Dumon C, Morland C, Gratien J, Heck MP, Stick RV, Blériot Y, Vasella A, Gilbert HJ, and Davies GJ. Structural and biochemical evidence for a boat-like transition state in beta-mannosidases. Nat Chem Biol. 2008 May;4(5):306-12. DOI:10.1038/nchembio.81 |
- Vincent F, Gloster TM, Macdonald J, Morland C, Stick RV, Dias FM, Prates JA, Fontes CM, Gilbert HJ, and Davies GJ. Common inhibition of both beta-glucosidases and beta-mannosidases by isofagomine lactam reflects different conformational itineraries for pyranoside hydrolysis. Chembiochem. 2004 Nov 5;5(11):1596-9. DOI:10.1002/cbic.200400169 |
- Hogg D, Pell G, Dupree P, Goubet F, Martín-Orúe SM, Armand S, and Gilbert HJ. The modular architecture of Cellvibrio japonicus mannanases in glycoside hydrolase families 5 and 26 points to differences in their role in mannan degradation. Biochem J. 2003 May 1;371(Pt 3):1027-43. DOI:10.1042/BJ20021860 |
- Tailford LE, Ducros VM, Flint JE, Roberts SM, Morland C, Zechel DL, Smith N, Bjørnvad ME, Borchert TV, Wilson KS, Davies GJ, and Gilbert HJ. Understanding how diverse beta-mannanases recognize heterogeneous substrates. Biochemistry. 2009 Jul 28;48(29):7009-18. DOI:10.1021/bi900515d |
- Cartmell A, Topakas E, Ducros VM, Suits MD, Davies GJ, and Gilbert HJ. The Cellvibrio japonicus mannanase CjMan26C displays a unique exo-mode of action that is conferred by subtle changes to the distal region of the active site. J Biol Chem. 2008 Dec 5;283(49):34403-13. DOI:10.1074/jbc.M804053200 |
- Caines ME, Vaughan MD, Tarling CA, Hancock SM, Warren RA, Withers SG, and Strynadka NC. Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the Apo and GM3 ganglioside-bound forms. J Biol Chem. 2007 May 11;282(19):14300-8. DOI:10.1074/jbc.M611455200 |
- Gloster TM, Ibatullin FM, Macauley K, Eklöf JM, Roberts S, Turkenburg JP, Bjørnvad ME, Jørgensen PL, Danielsen S, Johansen KS, Borchert TV, Wilson KS, Brumer H, and Davies GJ. Characterization and three-dimensional structures of two distinct bacterial xyloglucanases from families GH5 and GH12. J Biol Chem. 2007 Jun 29;282(26):19177-89. DOI:10.1074/jbc.M700224200 |
- Gebler J, Gilkes NR, Claeyssens M, Wilson DB, Béguin P, Wakarchuk WW, Kilburn DG, Miller RC Jr, Warren RA, and Withers SG. Stereoselective hydrolysis catalyzed by related beta-1,4-glucanases and beta-1,4-xylanases. J Biol Chem. 1992 Jun 25;267(18):12559-61.
- Wang Q, Tull D, Meinke A, Gilkes NR, Warren RA, Aebersold R, and Withers SG. Glu280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-beta-1,4-glucanase. J Biol Chem. 1993 Jul 5;268(19):14096-102.
- Dominguez R, Souchon H, Spinelli S, Dauter Z, Wilson KS, Chauvaux S, Béguin P, and Alzari PM. A common protein fold and similar active site in two distinct families of beta-glycanases. Nat Struct Biol. 1995 Jul;2(7):569-76.
- Ducros V, Czjzek M, Belaich A, Gaudin C, Fierobe HP, Belaich JP, Davies GJ, and Haser R. Crystal structure of the catalytic domain of a bacterial cellulase belonging to family 5. Structure. 1995 Sep 15;3(9):939-49.