Glycoside Hydrolase Family 89

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Glycoside Hydrolase Family GH89
Clan none
Mechanism Retaining
Active site residues known
CAZy DB link
http://www.cazy.org/GH89.html

Contents

Substrate specificities

Family 89 glycoside hydrolases are α-N-acetylglucosaminidases [1, 2]. The human lysosomal enzyme, NAGLU, is involved in the degradation of heparan sulfate [3, 4]. Mutations in this enzyme can cause a devastating disease called Sanfilippo syndrome type B which is also called mucopolysaccharidosis IIIB [1, 2, 3, 4].

Kinetics and Mechanism

Mechanistic and structural data is available on CpGH89, a family 89 glycoside hydrolase produced by Clostridium perfringens. CpGH89 uses a classical Koshland retaining mechanism to hydrolyze the glycosidic bond. This results in hydrolysis with a net retention of stereochemistry at the anomeric carbon [1].

Catalytic Residues

Two catalytically important glutamate residues have been identified in CpGH89, Glu483 and Glu601 [1]. These residues are between 6.1-6.7Å apart, which is consistent with a retaining catalytic mechanism. Mutation of Glu601 to an alanine results in an apparent abolishment of activity suggesting this residue is active as the catalytic nucleophile. Glu601 resides below the A-face of the sugar ring and is 2.8-3.1Å from C1 and appears suitably placed for nucleophilic attack on the anomeric carbon. Mutation of Glu483 to alanine results in much less severe impairments in catalysis suggesting this residue is active as the general acid/base residue. Glu483 is ~3.6Å from C1 and appears to be positioned in such a way that it would be capable of forming a hydrogen bond with the glycosidic oxygen of the substrate.

Three-dimensional structures

The three dimensional structure is available for CpGH89, see PDB entries 2vc9, 2vca, 2vcb and 2vcc [1]. CpGH89 is a multi-modular protein and quite large (2095 amino acids). Only residues 26-916 were crystallized. The N-terminal domain (residues 26-155) forms a β-sandwich fold and shares sequence identity to the family 32 carbohydrate-binding modules (CBMs). This module is tightly packed against the rest of the protein through a number of hydrophobic and hydrogen bonding interactions. The catalytic region is comprised of a small mixed α/β domain (residues 170-280), a decorated (α/β)8 core (residues 280-620), and an all α-helical domain (residues 621-916).

Family Firsts

First sterochemistry determination
1H NMR spectroscopy reveals that CpGH89 acts with retention of stereochemistry [1].
First catalytic nucleophile identification
The catalytic nucleophile was revealed by site directed mutagenesis on CpGH89 Glu601 [1].
First general acid/base residue identification
The general acid/base was revealed by site directed mutagenesis on CpGH89 Glu483 [1].
First 3-D structure
See PDB entries 2vc9, 2vca, 2vcb and 2vcc [1].

References

  1. Ficko-Blean E, Stubbs KA, Nemirovsky O, Vocadlo DJ, and Boraston AB. Structural and mechanistic insight into the basis of mucopolysaccharidosis IIIB. Proc Natl Acad Sci U S A. 2008 May 6;105(18):6560-5. DOI:10.1073/pnas.0711491105 | PubMed ID:18443291 | HubMed [1]
  2. Weber B, Hopwood JJ, and Yogalingam G. Expression and characterization of human recombinant and alpha-N-acetylglucosaminidase. Protein Expr Purif. 2001 Mar;21(2):251-9. DOI:10.1006/prep.2000.1361 | PubMed ID:11237686 | HubMed [2]
  3. Yogalingam G and Hopwood JJ. Molecular genetics of mucopolysaccharidosis type IIIA and IIIB: Diagnostic, clinical, and biological implications. Hum Mutat. 2001 Oct;18(4):264-81. DOI:10.1002/humu.1189 | PubMed ID:11668611 | HubMed [3]
  4. Weber B, Guo XH, Kleijer WJ, van de Kamp JJ, Poorthuis BJ, and Hopwood JJ. Sanfilippo type B syndrome (mucopolysaccharidosis III B): allelic heterogeneity corresponds to the wide spectrum of clinical phenotypes. Eur J Hum Genet. 1999 Jan;7(1):34-44. DOI:10.1038/sj.ejhg.5200242 | PubMed ID:10094189 | HubMed [4]
All Medline abstracts: PubMed | HubMed
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