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Glycoside Hydrolase Family 94

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Glycoside Hydrolase Family 94
Clan none (similar to GH-L)
Mechanism inverting
Active site residues known
CAZy DB link
http://www.cazy.org/fam/GH94.html

Substrate specificities

This family contains phosphorolytic enzymes (usually named using a combination of “the substrate” and “phosphorylase”) that cleave beta glycosidic bond. The substrate specificities found in GH94 are: cellobiose (Glc-β1,4-Glc) phosphorylase (EC 2.4.1.20), cellodextrin ((Glc-β1,4-)n-1Glc; n≥3) phosphorylase (EC 2.4.1.29), (N.N’-diacetyl)chitobiose (GlcNAc-β1,4;-GlcNAc) phosphorylase, and a domain phosphorolyzing protein-bound β-1,2-glucan accompanied by cyclic β1,2-glucan synthase(EC 2.4.1.-) belonging to GT84.


Phosphorylases

Kinetics and Mechanism

Catalytic Residues

Three-dimensional structures

Family Firsts

First sterochemistry determination
First catalytic nucleophile identification
First general acid/base residue identification
First 3-D structure
Cite some reference here, with a short explanation [1].

References

  1. Hidaka M, Honda Y, Kitaoka M, Nirasawa S, Hayashi K, Wakagi T, Shoun H, and Fushinobu S. (2004). Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold. Structure. 2004;12(6):937-47. DOI:10.1016/j.str.2004.03.027 | PubMed ID:15274915 [REF1]