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Glycoside Hydrolase Family 102

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Glycoside Hydrolase Family GHnn
Clan GH-x
Mechanism retaining
Active site residues known
CAZy DB link
http://www.cazy.org/fam/GHnn.html


Substrate specificities

Figure 1. Reaction catalyzed by family GH102 enzymes. LT, lytic transglycosylase. (click to enlarge).

The glycoside hydrolases of this family are lytic transglyosylases (also referred to as peptidoglycan lyases) of bacterial origin and they constitute family 2 of the classification scheme of Blackburn and Clarke [1]. The prototype for this family is membrane-bound lytic transglycosylase A (MltA) from Escherichia coli. These enzymes cleave the β-1,4 linkage between N-acetylmuramoyl and N-acetylglucosaminyl residues in peptidoglycan (Figure 1), but unlike the lytic transglycosylases of other families, they are active on peptidoglycan fragments lacking their stem peptides [2]. No other activities have been observed.


Kinetics and Mechanism

The lytic transglycosidases, strictly speaking, are retaining enzymes. However, they are not hydrolases but rather catalyse an intramolecular glycosyl transferase reaction onto the C-6 hydroxyl group of the muramoyl residue leading to the generation of a terminal 1,6-anhdyromuramoyl product (Figure 1) thus lacking a reducing end [3]. No detailed analyses involving both steady state and pre-steady state kinetic studies have been reported, but the Michaelis Menten (KM and Vmax) parameters have been estimated for E. coli MltA acting on insoluble peptidoglycan sacculi [2]

Catalytic Residues

Figure 2. Reaction mechanism proposed for E. coli MltA. (click to enlarge).

As with other lytic transglycosylases (families GH23, GH103, and GH104), the GH102 enzymes are thought to possess a single catalytic acid/base residue. This residue in E. coli MltA has been identified as Asp308 and, indeed, its replacement with Ala abolishes catalytic activity [4].

The mechanism of action of the family GH102 enzymes has yet to be proven experimentally, but examination of crystal structures of E. coli MltA complexed with chitohexoase has led to the proposal of a novel mechanism of action involving the stabilization of the putative transition state oxocarbenium ion by an alpha-helix dipole [5]. Thus, the catalytic Asp308 is proposed to serve initially as an acid catalyst to donate a proton to the glycosidic oxygen of the linkage to be cleaved leading to the formation of a transition intermediate with oxocarbenium ion character (Figure 2). In the absence of an anion/nucleophile in close proximity, this oxocarbenium intermediate is proposed to be stabilized by the negatively-charged side of the dipole that would exist at the C-terminal end of an alpha helix positioned just below the -1 binding subsite. This would be followed by abstraction of the C-6 hydroxyl proton of the intermediate involving Asp308 which now serves as the base catalyst leading to nucleophilic attack and the formation of 1,6-anhydromuramic acid product.

Three-dimensional structures

Three-dimensional structures are available for several Family GH102 enzymes, the first solved being that of E. coli MltA [4]. Unlike the other lytic transglycosylases of families GH23, GH103, and GH104 which possesses the well characterized α+β “lysozyme fold,” these enzymes have a unique structure consisting of two domains. One has a double-ψ β-barrel fold similar to the catalytic domain of the family GH45 endoglucanase V from Humicola insolens [6]. The second and smaller domain has a β-barrel fold topology. The large groove between the two domains serves as the active site cleft.


Family Firsts

First identification of lytic transglycosylase
MltA from E. coli [2].
First catalytic nucleophile identification
Not applicable for lytic transglycosylases.
First general acid/base residue identification
Inferred by X-ray crystallography of E. coli MltA [4].
First 3-D structure
E. coli MltA [4].
First identification as a lipoprotein
E. coli MltA [7].
First identification of localization to outer membrane
E. coli MltA [7].
Frist demonstration of molecular interactions between GH102 enzymes and penicillin-binding proteins
E. coli MltA [8].

References

  1. Blackburn NT and Clarke AJ. (2001). Identification of four families of peptidoglycan lytic transglycosylases. J Mol Evol. 2001;52(1):78-84. DOI:10.1007/s002390010136 | PubMed ID:11139297 [1]
  2. Ursinus A and Höltje JV. (1994). Purification and properties of a membrane-bound lytic transglycosylase from Escherichia coli. J Bacteriol. 1994;176(2):338-43. DOI:10.1128/jb.176.2.338-343.1994 | PubMed ID:8288527 [2]
  3. Höltje JV, Mirelman D, Sharon N, and Schwarz U. (1975). Novel type of murein transglycosylase in Escherichia coli. J Bacteriol. 1975;124(3):1067-76. DOI:10.1128/jb.124.3.1067-1076.1975 | PubMed ID:357 [3]
  4. van Straaten KE, Dijkstra BW, Vollmer W, and Thunnissen AM. (2005). Crystal structure of MltA from Escherichia coli reveals a unique lytic transglycosylase fold. J Mol Biol. 2005;352(5):1068-80. DOI:10.1016/j.jmb.2005.07.067 | PubMed ID:16139297 [4]
  5. van Straaten KE, Barends TR, Dijkstra BW, and Thunnissen AM. (2007). Structure of Escherichia coli Lytic transglycosylase MltA with bound chitohexaose: implications for peptidoglycan binding and cleavage. J Biol Chem. 2007;282(29):21197-205. DOI:10.1074/jbc.M701818200 | PubMed ID:17502382 [5]
  6. Davies GJ, Dodson GG, Hubbard RE, Tolley SP, Dauter Z, Wilson KS, Hjort C, Mikkelsen JM, Rasmussen G, and Schülein M. (1993). Structure and function of endoglucanase V. Nature. 1993;365(6444):362-4. DOI:10.1038/365362a0 | PubMed ID:8377830 [6]
  7. Lommatzsch J, Templin MF, Kraft AR, Vollmer W, and Höltje JV. (1997). Outer membrane localization of murein hydrolases: MltA, a third lipoprotein lytic transglycosylase in Escherichia coli. J Bacteriol. 1997;179(17):5465-70. DOI:10.1128/jb.179.17.5465-5470.1997 | PubMed ID:9287002 [7]
  8. Vollmer W, von Rechenberg M, and Höltje JV. (1999). Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli. J Biol Chem. 1999;274(10):6726-34. DOI:10.1074/jbc.274.10.6726 | PubMed ID:10037771 [8]

All Medline abstracts: PubMed

[[Category:Glycoside Hydrolase Families|GH102]