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Difference between revisions of "Glycoside Hydrolase Family 3"
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As more plant genome sequences are published, it is becoming clear that the GH3 glycoside hydrolases in plants are encoded by multi-gene families. In both monocot and dicot species, there are 15-18 genes for the GH3 enzymes, while in lower plants such as the Physcomitrella and Selaginella mosses 6-7 genes encoding GH3 glycoside hydrolases have been identified (<cite>Henrissat2001 GeislerLee2006</cite>, see also [http://www.phytozome.org/ Phytozome]). Plant β-''N''-acetylglucosaminidases have not been identified in GH3 thus far. | As more plant genome sequences are published, it is becoming clear that the GH3 glycoside hydrolases in plants are encoded by multi-gene families. In both monocot and dicot species, there are 15-18 genes for the GH3 enzymes, while in lower plants such as the Physcomitrella and Selaginella mosses 6-7 genes encoding GH3 glycoside hydrolases have been identified (<cite>Henrissat2001 GeislerLee2006</cite>, see also [http://www.phytozome.org/ Phytozome]). Plant β-''N''-acetylglucosaminidases have not been identified in GH3 thus far. | ||
+ | |||
+ | Due to the high diversity of protein structural arrangements found among GH3 members (see below), the phylogeny of this family is complex. Classification of GH3 members into subfamilies has been performed previously <cite>Harvey2000 Cournoyer2003</cite>, however a definitive subfamily classification (on par with those for [[GH13]], [[GH5]], and the polysaccharide lyases), is currently not available. | ||
== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
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#Henrissat2001 pmid=11554480 | #Henrissat2001 pmid=11554480 | ||
#Henrissat1991 pmid=1747104 | #Henrissat1991 pmid=1747104 | ||
+ | #Cournoyer2003 pmid=12766348 | ||
</biblio> | </biblio> | ||
[[Category:Glycoside Hydrolase Families|GH003]] | [[Category:Glycoside Hydrolase Families|GH003]] |
Revision as of 09:31, 18 April 2013
This page has been approved by the Responsible Curator as essentially complete. CAZypedia is a living document, so further improvement of this page is still possible. If you would like to suggest an addition or correction, please contact the page's Responsible Curator directly by e-mail.
- Authors: ^^^Geoff Fincher^^^, ^^^Brian Mark^^^, and ^^^Harry Brumer^^^
- Responsible Curator: ^^^Bernard Henrissat^^^
Glycoside Hydrolase Family GH3 | |
Clan | none |
Mechanism | retaining |
Active site residues | known |
CAZy DB link | |
http://www.cazy.org/GH3.html |
Substrate specificities
The GH3 glycoside hydrolase family currently group together exo-acting β-D-glucosidases, α-L-arabinofuranosidases, β-D-xylopyranosidases and N-acetyl-β-D-glucosaminidases [1]. Widely distributed in bacteria, fungi and plants, GH3 enzymes carry out a range of functions including cellulosic biomass degradation, plant and bacterial cell wall remodeling, energy metabolism and pathogen defense. In many cases the enzymes have dual or broad substrate specificities with respect to monosaccharide residues, linkage position and chain length of the substrate. For example, there are several well-characterized ‘bifunctional’ enzymes in the family that have both α-L-arabinofuranosidase and β-D-xylopyranosidase activity [2], and one characterized example of an N-acetyl-β-D-glucosaminidase/β-glucosidase from Cellulomonas fimi (Nag3) [3]. GH3 β-D-glucosidases from barley, which are more precisely referred to as β-D-glucan glucohydrolases, are also broad specificity exo-hydrolases that remove single glucosyl residues from the non-reducing ends of a range of β-D-glucans, β-D-oligoglucosides and aryl β-D-glucosides, including (1,3)-β-D-glucans, (1,4)-β-D-glucans, (1,3;1,4)-β-D-glucans and (1,6)-β-D-glucans, 4-nitrophenyl β-D-glucoside, certain cyanogenic β-D-glucosides and some β-D-oligoxyloglucosides [4].
In contrast to the broad substrate specificities observed for the GH3 enzymes described above, GH3 N-acetyl-β-D-glucosaminidases are selective for N-acetyl-β-D-glucosamine (GlcNAc) [5] (though exceptions exist such as Cellulomonas fimi Nag3 [3]). A notable GH3 N-acetyl-β-D-glucosaminidase of prokaryotes is NagZ, which participates in bacterial cell wall recycling (for review see [6]) by removing GlcNAc from 1,6-anhydroMurNAc-peptides in Gram-negative bacteria [7], or GlcNAc from GlcNAc-MurNAc-peptides in Gram-positive bacteria [8]. The NagZ product 1,6-anhydroMurNAc-peptide is an important activator of AmpC β-lactamase hyper-production in several Gram-negative pathogens, making the enzyme of interest as a potential therapeutic target [9].
As more plant genome sequences are published, it is becoming clear that the GH3 glycoside hydrolases in plants are encoded by multi-gene families. In both monocot and dicot species, there are 15-18 genes for the GH3 enzymes, while in lower plants such as the Physcomitrella and Selaginella mosses 6-7 genes encoding GH3 glycoside hydrolases have been identified ([10, 11], see also Phytozome). Plant β-N-acetylglucosaminidases have not been identified in GH3 thus far.
Due to the high diversity of protein structural arrangements found among GH3 members (see below), the phylogeny of this family is complex. Classification of GH3 members into subfamilies has been performed previously [1, 12], however a definitive subfamily classification (on par with those for GH13, GH5, and the polysaccharide lyases), is currently not available.
Kinetics and Mechanism
![](/images/7/75/GH3_Fig_1.png)
GH3 glycoside hydrolases remove single glycosyl residues from the non-reducing ends of their substrates. Catalysis occurs via a classical Koshland double-displacement mechanism with the anomeric configuration of the released glycose being retained. The retention of anomeric configuration has been established experimentally for several enzymes (Which? And how?). The active site of GH3 enzymes consists of two glucosyl-binding subsites (-1 and +1) with an enzymic nucleophile and general acid/base residue flanking the junction of these two subsites (see [13] for subsite nomenclature). This arrangement of catalytic residues was first visualized in 1999 when the crystal structure of barley β-D-glucan glucohydrolase was determined in complex with glucose [14] (Fig. 1).
Several studies have contributed to the understanding of the kinetics and mechanism of GH3 enzymes, including detailed analyses of β-glucosidases from fungi (Aspergillus wentii [15] and Aspergillus niger [16, 17]) and Gram-negative bacteria (Flavobacterium meningosepticum [18, 19], Thermotoga neapolitana [20]), as well as a GH3 glucosylceramidase from the Gram-positive microbe Paenibacillus sp. TS12 [21]. Kinetic and mechanistic analyses of β-D-glucan glucohydrolases and two ‘bifunctional’ α-L-arabinofuranosidase/β-D-xylopyranosidases from plants (barley) have also been carried out [2, 4]. These studies, combined with kinetic and mechanistic analyses of N-acetyl-β-D-glucosaminidases from the Gram-positive microbe Bacilus subtillus [8, 22], and Gram-negative microbes Vibrio furnisii [23, 24], Vibrio cholerea [25] and Salmonella typhimerium [22] confirm that while the catalytic nucleophile of GH3 enzymes is well conserved, the location and identity of the general acid/base residue is not.
Catalytic Residues
Catalytic nucleophile
![](/images/thumb/9/96/GH3_2013_Fig3.png/400px-GH3_2013_Fig3.png)
![](/images/thumb/5/50/GH3_2013_Fig4.png/400px-GH3_2013_Fig4.png)
![](/images/thumb/4/4e/GH3_2013_Fig5.png/400px-GH3_2013_Fig5.png)
Early labeling experiments of a β-glucosidase from Aspergillus wentii using conduritol B-epoxide by Bause and Legler in 1974 [15] suggested an aspartate residue within the sequence VMSDW as the putative catalytic nucleophile long before the establishment of the CAZy classification [27] and recognition of this enzyme as a GH3 member. The homologous residue was later implicated by the crystal structure of barley β-D-glucan glucohydrolase, which identified Asp285 within the sequence GFVISDW as being appropriately positioned to act as a nucleophile during catalysis [14]. Direct identification of this aspartate as the catalytic nucleophile via active-site labeling using 2-deoxy-2-fluoro-β-D-glycosides of GH3 β-glucosidases from A. niger [16] and F. meningosepticum [18], a glucosylceramidase from Paenibacillus sp. [21], as well as the barley β-D-glucan glucohydrolase (including conduritol B-epoxide labelling and crystallography of both inactive complexes) [28], and labeling of NagZ enzymes from V. furnisii [23] and S. typhimerium [22] using 2-acetamido-2-deoxy-5-fluoro-β-D-glucopyranosyl fluoride, have confirmed that this catalytic residue is well conserved in GH3 enzymes from diverse species. The conservation of this aspartate becomes clear when known crystal structures of a number of GH3 enzymes are superposed (Fig. 2).
Catalytic acid/base
Though sequence alignments can now be used to easily identify the catalytic nucleophile of GH3 members, the position of the general acid/base residue is phylogenetically variable and less readily identifiable [17]. Detailed kinetic analyses and an increase in 3D structural information continue to provide insight into identity of the elusive general acid-base residue across the diversity of GH3 members.
The seminal structural study of the barley β-D-glucan glucohydrolase ExoI, in complex with the product glucose, was the first to suggest the identity of the catalytic acid/base in a GH3 member. Barley ExoI is a two-domain enzyme consisting of an N-terminal (β/α)8 barrel domain housing the active site pocket and the catalytic nucleophile (Asp285). The C-terminal domain contains a glutamate residue (Glu 491) projecting into the active site of the (β/α)8 barrel domain, which was proposed to act as the catalytic acid/base [14]. These assignments were also supported by crystallography of pseudo-Michaelis complexes with non-hydrolyzable thio-glycosides [28, 29].
Kinetic analyses, including the use of site-directed mutation and chemical rescue, have been used to provide early experimental support for the identification of the catalytic acid/base in several bacterial and fungal GH3 members: Flavobacterium meningosepticum β-glucosidase [19], Paenibacillus sp. TS12 glucosylceramidase [21], Thermotoga neapolitana β-glucosidase [20], and Aspergillus niger β-glucosidase [17]. The identification of Glu-473 as the catalytic acid/base in the F. meningosepticum enzyme was further supported by covalent labelling with N-bromoacetyl-β-D-glucosylamine and peptide mass spectrometry [18].
Later structural studies of bacterial and fungal GH3 enzymes have since shown that while insertion of additional domains is possible (which shifts the location of the glutamic acid general acid-base within the primary sequence), the contribution of two-domains to the active site architecture, as first observed for the barley enzyme, appears to be a core feature of multidomain GH3 β-glucanases [20, 30, 31]. Superposition of available GH3 β-D-glucan glucohydrolase structures clearly reveals the conservation of this architecture, and directly highlights active-site residue homology in the absence of protein sequence similarity - especially so in the case of the catalytic acid-base [17] (Fig. 2).
Notably, GH3 NagZ enzymes represent a significant departure from the above two-domain active site architecture paradigm. A crystal structure of NagZ from B. subtilis, together with kinetic analysis, provided evidence that the catalytic acid-base is an unusual histidine/aspartate dyad that resides within a flexible loop on the catalytic (β/α)8 barrel, not on a separate domain [26]. Though the enzyme adopts a two-domain fold similar to the barely β-D-glucan glucohydrolase, the C-terminal domain does not participate in catalysis (Fig. 3). In fact, most NagZ enzymes from Gram-negative bacteria are single domain enzymes comprised solely of a catalytic (β/α)8 barrel that contains the conserved aspartate nucleophile and a catalytic histidine/aspartate dyad on flexible loop, as seen for B. subtilis NagZ [22] (Fig. 4). The flexible loop containing the His/Asp dyad is unique to GH3 NagZ enzymes and can be identified by the consensus motif [KH(F/I)PG(H/L)GXXXXD(S/T)H] (catalytic dyad highlighted in boldface) [23]. In light of these studies, the residue identification in a Clostridium paraputrificum N-acetyl-β-D-glucosaminidase (Nag3A) would appear to be unreliable [32].
Three-dimensional structures
![](/images/thumb/8/83/GH3_2013_Fig6.png/400px-GH3_2013_Fig6.png)
Crystal structures are now available for a number of multidomain GH3 members, including barley β-D-glucan glucohydrolase [14] and a mechanistically related β-glucanase from the marine bacterium Pseudoalteromonas sp. [30], as well as β-glucosidases from Kluyveromyces marxianus (KmBglI) [31] , Trichoderma reesei (Cel3A) (PDB 4I8D (unpublished)), Thermotoga neapolitana [20] and a macrolide β-glycosidase / β-glucosidase (DesR) from Streptomyces venezuelae [33] (please see the GH3 structure page of the CAZy DB for a continuously updated list). These structures have revealed considerable diversity in architecture and arrangement of domains in GH3 enzymes that are present in addition to the core catalytic domain, and have provided insight into how these domains affect function.
Crystal structures of a number of NagZ enzymes are also available, including the two-domain NagZ from B. subtilis [26] and single domain NagZ enzymes from Vibrio cholera (PDB 1TR9 (unpublished)) & [25], S. typhimurium [22], Deinococcus radiodurans (PDB 3TEV (unpublished)) and Burkholderia cenocepacia (PDB 4GNV (unpublished)). Given the role of NagZ in the activation of AmpC-mediated β-lactam resistance in Gram-negative bacteria [9], a number of crystallographic and kinetic studies have focused on the development of small-molecule inhibitors of NagZ [25, 34, 35], some of which have been designed to be selective for GH3 NagZ over functionally related human enzymes from families GH20 and GH84 [25, 34].
Insight into the broad specificity observed for some GH3 enzymes has been provided from X-ray crystallographic data and from molecular modeling of enzyme-substrate complexes of the barley β-D-glucan glucohydrolase [14, 29]. Recent structural studies of NagZ enzymes from S. typhimurium and B. subtilis have shown the flexible catalytic loop participates in distorting the bound terminal GlcNAc sugar toward a 1S3 conformation during catalysis [22] (Fig. 5). Distortion of the substrate toward a 1S3 conformation is in agreement with other retaining β-glycosidases; however, this has not been observed for other GH3 enzymes, in which the substrate has been found in a relaxed, near-4C1 conformation.
Family Firsts
- First sterochemistry determination
- To be added.
- First catalytic nucleophile identification
- First suggested by Bause and Legler in 1974 using conduritol B-epoxide labelling of an Aspergillus wentii glucosidase [15], and later supported by the crystal structures of a product complex of a barley β-D-glucan glucohydrolase [14] and a trapped covalent intermediate [28]. Contemporaneous active-site labeling of an A. niger β-glucosidase [16] and V. furnisii NagZ [23] using 2-deoxy-2-fluoro-β-D-glycosides allowed unequivocal identification of the catalytic nucleophiles in these enzymes.
- First general acid/base identification
- In contrast to the catalytic nucleophile, the catalytic acid/base is not broadly conserved on the protein sequence level; thus, the "first" identification was not strictly definitive in this family (this living history is detailed above). The GH3 general acid/base was first suggested by a product complex of a barley β-D-glucan glucohydrolase [14] (and later by other complex structures of this same enzyme [28, 29]). The earliest definitive kinetic studies were performed on a Flavobacterium meningosepticum β-glucosidase [18, 19]. The first revelation of the atypical Asp/His dyad fulfilling this role was for B. subtilis NagZ [8].
- First 3-D structure
- The first 3D structure in family GH3 was that of the two-domain barley β-D-glucan glucohydrolase [14]. As discussed above, the number and organization of domains among GH3 members is diverse, such that a number of seminal structures could be highlighted.
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