CAZypedia needs your help!
We have many unassigned pages in need of Authors and Responsible Curators. See a page that's out-of-date and just needs a touch-up? - You are also welcome to become a CAZypedian. Here's how.
Scientists at all career stages, including students, are welcome to contribute.
Learn more about CAZypedia's misson here and in this article.
Totally new to the CAZy classification? Read this first.

Glycoside Hydrolase Family 54

From CAZypedia
Jump to navigation Jump to search

Glycoside Hydrolase Family 54
Clan none
Mechanism retaining
Active site residues known
CAZy DB link
http://www.cazy.org/fam/GH54.html

Substrate specificities

This family contains α-L-arabinofuranosidase (EC 3.2.1.55) and β-xylosidase (EC 3.2.1.37).


Kinetics and Mechanism

GH54 members are retaining enzymes.


Catalytic Residues

Three-dimensional structures

The first solved 3-D structure was α-L-arabinofuranosidase B (AkAbfB) from Aspergillus kawachii IFO 4308 (http://www.rcsb.org/pdb/explore/explore.do?structureId=1WD3 PDB 1wd3) in 2004 [1].

Carbohydrate-Binding Module

Most of the members in GH54 have Carbohydrate-Binding Module Family 42 of approx. 160 residues at the C-terminus of GH54 catalytic domains. Binding to arabinofuranose (present in arabinoxylan) has been demonstrated [2].

Family Firsts

First sterochemistry determination
Cite some reference here, with a short explanation.
First catalytic nucleophile identification
First general acid/base residue identification
First 3-D structure
α-L-Arabinofuranosidase B (AkAbfB) from Aspergillus kawachii IFO 4308 (http://www.rcsb.org/pdb/explore/explore.do?structureId=1WD3 PDB 1wd3) [1].

References

  1. Miyanaga A, Koseki T, Matsuzawa H, Wakagi T, Shoun H, and Fushinobu S. (2004). Crystal structure of a family 54 alpha-L-arabinofuranosidase reveals a novel carbohydrate-binding module that can bind arabinose. J Biol Chem. 2004;279(43):44907-14. DOI:10.1074/jbc.M405390200 | PubMed ID:15292273 [REF1]
  2. Miyanaga A, Koseki T, Miwa Y, Mese Y, Nakamura S, Kuno A, Hirabayashi J, Matsuzawa H, Wakagi T, Shoun H, and Fushinobu S. (2006). The family 42 carbohydrate-binding module of family 54 alpha-L-arabinofuranosidase specifically binds the arabinofuranose side chain of hemicellulose. Biochem J. 2006;399(3):503-11. DOI:10.1042/BJ20060567 | PubMed ID:16846393 [REF2]

All Medline abstracts: PubMed