Polysaccharide Lyase Family 30

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• Author: Marie-Line Garron
• Responsible Curator: Marie-Line Garron

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Polysaccharide Lyase Family PL30
3D Structure
Mechanism
Charge neutraliser
Active site residues
CAZy DB link
https://www.cazy.org/PL30.html

Substrate specificities

PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum Bacteroidetes. The founding member of family 30, and the only one characterized, belongs to Bacteroides cellulosilyticus WH2 (BcellWH2_02524, WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that BcellWH2_02524 is a hyaluronan lyase (4.2.2.1), probably using the by β-elimination process like all PL families [1].

Kinetics and Mechanism

Not Determined

Catalytic Residues

Not Determined

Three-dimensional structures

Unsolved

Family Firsts

First description of catalytic activity

Hyaluronan lyase was the first activity discovered for the family PL30 [1].

First charge neutralizer identification

To be determined

First Brønstead acid and base residue identification

To be determinated

First 3-D structure

To be determinated

References

1. Helbert W, Poulet L, Drouillard S, Mathieu S, Loiodice M, Couturier M, Lombard V, Terrapon N, Turchetto J, Vincentelli R, and Henrissat B. Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space. Proc Natl Acad Sci U S A. 2019 Mar 26;116(13):6063-6068. DOI:10.1073/pnas.1815791116 |